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KMID : 1007519990080040227
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Food Science and Biotechnology
1999 Volume.8 No. 4 p.227 ~ p.231
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Measurement of the Estrogenic Activity of Zearalenone in Extruded Corn Grits by Cell Culture Technique
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Ryu Do-Jin
Lloyd B. Bullerman
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Abstract
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An in vitro bioassay method using a human breast cancer cell line (MCF-7) and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) technique was developed to monitor the estrogenic activity of zearalenone. Dose related growth of MCF-7 was a measure of estrogenic activity. Zearalenone showed a clear proliferative effect on MCF-7 when measured by MTT, possibly due to the presence of estrogen-specific receptors in the cell line. Relative estrogenic activity of zearalenone was determined to be 4% of that of 17β-estradiol, which is consistent with those of previous investigations. Food grade corn grits were spiked with culture material containing a high level of zearalenone and processed with a laboratory scale twin screw extruder. Estrogenic potentials of residual zearalenone in the collected samples were analyzed. The established cell culture-MTT assay was determined to be effective in assessing estrogenic activity in extruded corn grits.
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